The work is devoted to the study of ways to preserve and propagate the most valuable plant species of the Tomsk flora in the conditions of the Siberian Botanical Garden of TSU. This problem has been solved by us for over 30 years. More than 200 species have been tested, including about 100 rare and protected species of local flora. The objects of research are rare and endangered plant species of the Tomsk region, which are grown in the areas of the experimental economy of SibBS TSU.