This is Vol. 2 of Bioinformatics Algorithms: an Active Learning Approach, one of the first textbooks to emerge from the recent Massive Open Online Course (MOOC) revolution. A light-hearted and analogy-filled companion to the authors' acclaimed Bioinformatics Specialization on Coursera (http:coursera.orgspecializationbioinformatics34), this book presents students with a dynamic approach to learning bioinformatics. It strikes a unique balance between practical challenges in modern biology and fundamental algorithmic ideas, thus capturing the interest of students of both biology and computer science.