An Introduction to Assembly Programming with RISC-V is a textbook for programmers that want to learn how to program in assembly language. It leverages the RISC-V ISA to guide the reader through the main concepts of assembly programming and the assembly process. The book is divided into three parts: I) Introduction to computer systems and assembly language, II) User-level programming, and III) System-level programming. The first part, "Introduction to computer systems and assembly language", starts by providing a brief overview of how computers are organized and how programs are executed by computers. Then, it discusses how data is represented on modern computers, including how numbers and text are represented, how data is organized on the main memory, and how computer instructions are encoded. Next, it discusses how native programs are generated, the assembly process, and the main elements of object (binary) files, including symbols, labels, and references. Finally, it introduces the main elements of the assembly language and some of the most common assembly directives. This book is intended for people that have basic knowledge of computer programming using high-level languages and want to learn assembly programming.